Protein G Agarose Beads from Cell Signaling Technology

Product Specs
ItemProtein G Agarose Beads
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number37478P
Quantity200 µl
ApplicationsIP
Research AreaRelated Products

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Characterisation of forkhead box protein A3 as a key transcription factor for hepatocyte regenerationJHEP ReportsDecember 1, 2023Guoqiang Li, Lijun Zhu, Mingwei Guo, Dongmei Wang, Meiyao Meng, Yinzhao Zhong, Zhijian Zhang, Yi Lin, Caizhi Liu, Jiawen Wang, et al.Bioruptor sonicator (Scientz, Ningbo, China) was used to fragment chromatin. Samples were incubated with Protein G Agarose beads and then immunoprecipitated with IgG (2729S; Cell Signaling Technology), anti-FOXA3 antibody (sc-74424;

(2) RAR-Dependent and RAR-Independent RXR Signaling in Stem-like Glioma CellsInternational Journal of Molecular SciencesNovember 17, 2023Amanda Dabrock, Natalie Ernesti, Florian Will, Manaf Rana, Nadja Leinung, Phillip Ehrich, Volker Tronnier, Christina ZechelImmunoprecipitation was preceded by a preclearing step (2–4 h on a rotator at 4 °C) using a mixture of Protein A and Protein G agarose beads (Cell Signaling). The chromatin was incubated for 4 h with the antibodies before capture was carried

(3) Transcriptional repression of beige fat innervation via a YAP/TAZ-S100B axisNature CommunicationsNovember 4, 2023Xun Huang, Xinmeng Li, Hongyu Shen, Yiheng Zhao, Zhao Zhou, Yushuang Wang, Jingfei Yao, Kaili Xue, Dongmei Wu, Yifu Qiuprimary antibodies at 4 °C overnight and then added with Protein G Beads (Cell Signaling Technology, 37478) for 3 h at 4 °C. The beads were washed with immunoprecipitation lysis buffer for three times and the

(4) H3K4me3 remodeling induced acquired resistance through O-GlcNAc transferase☆Drug Resistance UpdatesNovember 1, 2023Dinoop Ravindran Ravindran Menon, Heinz Hammerlindl, Gregory Gimenez, Sabrina Hammerlindl, Elmar Zuegner, Joachim Torrano, Natalie Bordag, Abdullah Al Al Emran, Maybelline Giam, Simon Denil, et al.sonicated on ice three times for 5 s. Cell lysates were subject to a pre-cleaning step by incubating with Protein G Agarose Beads (Cell Signaling) for 1 h at 4°C with gentle rocking. Pre-cleaned cell lysates corresponding to 500 μg

(5) Activation of AIM2 by hepatitis B virus results in antiviral immunity that suppresses hepatitis C virus during coinfectionJournal of VirologyOctober 31, 2023Yongqi Li, Yang Yang, Tianyang Li, Zhengmin Wang, Chunfeng Gao, Rilin Deng, Faxiang Ma, Xinyang Li, Licong Ma, Renyun Tian, et al.buffer. AIM2 or NLRP3 immunoprecipitation was performed by adding Protein G Agarose beads (Catalog: 37478, Cell Signaling Technology) and corresponding antibody to the lysates. After overnight incubation at

(6) Global lactylome reveals lactylation-dependent mechanisms underlying T H 17 differentiation in experimental autoimmune uveitisScience AdvancesOctober 20, 2023Wei Fan, Xiaotang Wang, Shuhao Zeng, Na Li, Guoqing Wang, Ruonan Li, Siyuan He, Wanqian Li, Jiaxing Huang, Xingran Li, et al.micrococcal nuclease and then incubated with anti-Ikzf1 (CST, 14859) or anti-IgG (CST, 3900) antibodies and Protein G Agarose beads overnight at 4°C. Then, the DNA was purified for further experiments. Luciferase activity assays The

(7) Single-cell signaling analysis reveals that Major Vault Protein facilitates RasG12C inhibitor resistancebioRxiv : the preprint server for biologyOctober 4, 2023Zhang JZ, Ong SE, Baker D, Maly DJ.Spectra™ Multicolor High Range Protein Ladder,Thermo Fisher,26625 Protein A Agarose Beads,CST,9863S Protein G Agarose Beads,CST,37478 Puromycin,Tocris,4089 Hygromycin,Tocris,4137 DPBS,Gibco,14190250 Doxycycline,Tocris,4090 PEI Max,PolyScien

(8) GDNF triggers proliferation of rat C6 glioma cells via the NF-κB/CXCL1 signaling pathwayPLoS OneAugust 18, 2023Yue Wang, Yue Wu, Li Li, Jin Gao, Dian Shuai Gao, Shen Suncontrol. Immunoprecipitation was carried out overnight at 4°C and immune complexes were collected using Protein G Agarose beads. The immune complexes and the input were eluted and protein-DNA complexes were de-crosslinked overnight

(9) Diabetes impairs fracture healing through Foxo1 mediated disruption of ciliogenesisCell Death DiscoveryAugust 17, 2023Zahra Chinipardaz, Gongsheng Yuan, Min Liu, Dana T. Graves, Shuying YangSignaling, 2880S) or normal rabbit IgG (Cell Signaling, 2729). Protein-DNA complexes were purified using protein G Agarose beads (Cell Signaling, 9007). RT-qPCR was performed with IFT80 promoter primers [IFT80F (5′-TGGTCGCAGGACAGCCTTTG-3′)

(10) Activating transcription factor 4 drives the progression of diabetic cardiac fibrosisESC Heart FailureAugust 1, 2023Yu Li, Qian He, Chao‐Yong He, Chao Cai, Zhen Chen, Jing‐Zhu Duan(#3900, Cell Signaling Technology) or anti‐Smurf2 (#12024, Cell Signaling Technology) and incubated with protein G‐Agarose beads (Yeasen, Shanghai, China) at 4°C overnight. Subsequently, washing with 1 mL lysis buffer was performed

(11) Mitotic phosphorylation of Tau/MAPT modulates cell cycle progression in prostate cancer cellsJournal of Cancer Research and Clinical OncologyAugust 1, 2023Letizia Clementi, Samantha Sabetta, Veronica Zelli, Chiara Compagnoni, Alessandra Tessitore, Vincenzo Mattei, Adriano AngelucciImmunoprecipitation assay Anti β-tubulin antibody (D20G3) (Cell Signalling Technology) was incubated for 2 h at 4 °C with protein-G Agarose Beads (Cell Signalling Technology) and Protein A-Agarose Beads (Santa Cruz Biotechnology) in Tris 50 mM pH

(12) The Fe-S cluster assembly protein IscU2 increases α-ketoglutarate catabolism and DNA 5mC to promote tumor growthCell discoveryJuly 25, 2023Ren X, Yan J, Zhao Q, Bao X, Han X, Zheng C, Zhou Y, Chen L, Wang B, Yang L, et al.antibodies (Cell Signaling Technology) according to the manufacturer’s suggestion. The next day, 30 μL of protein G agarose beads was added to each sample, and the immunoprecipitation mixture was washed with low- and high-salt washes.

(13) Reduced acetylation of TFAM promotes bioenergetic dysfunction in the failing heartiScienceJune 16, 2023Manling Zhang, Ning Feng, Zishan Peng, Dharendra Thapa, Michael W. Stoner, Janet R. Manning, Charles F. McTiernan, Xue Yang, Michael J. Jurczak, Danielle Guimaraes, et al.antibody (Cell Signaling Technology #9441) and protein G agarose beads (Cell Signaling Technology #37478) overnight at 4°C. Beads were washed 4 times with RIPA buffer, and then eluted directly with LDS sample

(14) The regulation of Tfh cell differentiation by β-hydroxybutyrylation modification of transcription factor Bcl6ChromosomaMay 25, 2023Jingtian Guo, Yimeng Wang, Lei Tang, Tiejun Tang, Zhuolan Li, Mengyuan Li, Liming Wang, Aizhong Zeng, Yuxiao Ma, Shihao Huang, et al.Anti-β-hydroxybutyryllysine antibody (PTM-1204) was from PTMbio (China). Protein A/G-PLUS-agarose beads (37478) were from Cell Signaling Technology (Danvers, USA). Fluorochrome-conjugated anti-CD4 (RPA-T4), anti-human

(15) Bre1/RNF20 promotes Rad51-mediated strand exchange and antagonizes the Srs2/FBH1 helicasesNature communicationsMay 25, 2023Liu G, Li J, He B, Yan J, Zhao J, Wang X, Zhao X, Xu J, Wu Y, Zhang S, et al.(Cell Signaling Technology; Rabbit; #5546; 1:200 dilution) antibody overnight at 4 °C, followed by incubation with protein G-agarose beads (Cytiva, #17061805) for 4 h at 4 °C. The protein-bound beads were washed twice with lysis buffer, twice

(16) Overcoming adaptive resistance to anti-VEGF therapy by targeting CD5LNature CommunicationsApril 26, 2023Christopher J. LaFargue, Paola Amero, Kyunghee Noh, Lingegowda S. Mangala, Yunfei Wen, Emine Bayraktar, Sujanitha Umamaheswaran, Elaine Stur, Santosh K. Dasari, Cristina Ivan, et al.immunoprecipitation with PPARG (Cell Signaling) antibody or IgG control. Immunocomplexes were collected with protein G agarose beads and eluted. Cross-links were reversed by incubating at 65 °C. DNA was then extracted and purified for

(17) RBM14 as a novel epigenetic-activated tumor oncogene is implicated in the reprogramming of glycolysis in lung cancerWorld Journal of Surgical OncologyApril 14, 2023Yan Hu, Hanshuo Mu, Zhiping Dengthe cell lysates were incubated with indicated antibody and protein A/G PLUS agarose beads (#9863, #37478, Cell Signaling Technology) overnight at 4 °C. After washing, the beads were lysed with loading buffer

(18) EMT-activated secretory and endocytic vesicular trafficking programs underlie a vulnerability to PI4K2A antagonism in lung cancerThe Journal of clinical investigationApril 3, 2023Tan X, Xiao GY, Wang S, Shi L, Zhao Y, Liu X, Yu J, Russell WK, Creighton CJ, Kurie JM.Signaling Technology), and incubated with antibodies at 4°C overnight. The immune complex was captured with Protein G Agarose Beads (Cell Signaling Technology), washed with 1× RIPA buffer once and 1× PBS thrice, and boiled in 1× sodium

(19) Microtubule-associated proteins MAP7 and MAP7D1 promote DNA double-strand break repair in the G1 cell cycle phaseiScienceMarch 17, 2023Dullovi A, Ozgencil M, Rajvee V, Tse WY, Cutillas PR, Martin SA, Hořejší Z.A2220 GFP-trap agarose,Antibodies Online (ChromoTek),Cat# ABIN509398 Protein G agarose,Cell Signalling,Cat# 37478 λ-phosphatase,NEB,Cat# P0753L pBRCA1: Biotin-eahx-QSESQGVGLpSDKELVSDDEE-amide,The Francis Crick protein

(20) Mechanical force induces macrophage-derived exosomal UCHL3 promoting bone marrow mesenchymal stem cell osteogenesis by targeting SMAD1Journal of nanobiotechnologyMarch 14, 2023Pu P, Wu S, Zhang K, Xu H, Guan J, Jin Z, Sun W, Zhang H, Yan B.Cell Signaling Technology), anti-SMAD1 (#AP20642c, Abcepta) or IgG (#A7028, Bytotime) antibodies at 4 °C for 2 h. Then 60 μl protein-G agarose beads were added, and the mixture was incubated with rocking at 4 °C overnight. After the agarose beads were

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Protein G Agarose Beads from Cell Signaling Technology

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